The purpose of this study is to evaluate an effect of levamisole on the chemical carcinogenesis in the submandibular salivary gland of rats through histopathologic observation.
60 male Sprague Dawley rats were employed in this study, divided into one control and two experimental groups. An pellet of 5 mg of 9, 10-dimethyl-1, 2-benzanthracene(DMBA) powder was implanted into submandibular salivary gland of each animal among 20 in control. And each animal among 20 in experimental group I received 0.7 mg of levamisole hydrochloride orally every day starting at the beginning of the fifth week after DMBA implantation under the same method as in control. And each animal among 20 in experimental group 2 received the same treatment as in control at the beginning of the fifth week after oral administration of levamisole hydrochloride under the same method as experimental group Each 5 animals in -control at the end of 2nd, 4th, 6th, 8th, week after experiments, and each .10 animals in experimental group 1. 2 at the end of 6th, 8th week after experiments were sacrificed at random. Also the specimens from experimental sites of submandibular salivary glandss were routinely processed for histopathologic observation under hematoxylin-eosin(H-E) staining.. The obtained results were as follows :
1. In control, generally, the glandular ductal cell showed the tendency of dysplasia or malignancy with progression of experiment.
2. In experimental group 1, generally, the dysplasia or malignancy of the glandular ductal cell was less prominent than in control, while the lymphocyte infiltration-and fibrosis were prominent.
3. In experimental group 2, generally, the dysplasia of the glandular ductal cell was signif, cantly less prominent than in control, while the fibrosis was prominent.
4. Under above results levamisole was thought to delay or prevent the chemical carcinogenesis in the submandibular salivary gland.
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